Human SDF-1 alpha ELISA Kit

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Human SDF-1 alpha ELISA Kit
Human SDF-1 alpha ELISA Kit Standard Curve

Human SDF-1 alpha ELISA Kit

Catalog No : 3010136


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SKU: 3010136. Category: .

Human SDF-1 alpha ELISA Kit Features

Product Name            Human SDF-1 alpha ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   40 pg/ml
Assay Range               93.8-6000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CXCL12, IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
Gene ID                        6387
Swissprot ID              P48061

 

Recovery

Sample Type    Average % RecoveryRange %
Plasma9083-102
Cell culture media9484-107

Linearity

Sample TypeDilution% of Expected
Plasma1:293
1:495
1:896
Cell culture media1:295
1:4101
1:894

Standard Curve: Human SDF-1 alpha ELISA Kit

Human SDF-1 alpha ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells. Stromal cell-derived factors 1-alpha and 1-beta are activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF, or IL1.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human SDF-1 alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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