Human SDF-1 alpha EasyTest™ ELISA Kit

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Human SDF-1 alpha EasyTest ELISA Kit Standard Curve

Human SDF-1 alpha EasyTest™ ELISA Kit

Catalog No : 1010136


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SKU: 1010136. Category: .

Product Features

Product Name           Human SDF-1 alpha EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   7 pg/mL
Assay Range                25-1600pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Cxcl12, Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
Gene ID                         6387
Swissprot ID               P48061

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9283-101
Plasma9485-110

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:492
1:889
Plasma1:2106
1:494
1:892

Standard Curve: Human SDF-1 alpha EasyTest™ ELISA Kit

Human SDF-1 alpha EasyTest ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human SDF-1 alpha mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

CXCL12 is expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. It has also been shown that CXCL12 signalling regulates the expression of CD20 on B cells. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human SDF-1 alpha EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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