Human PF-4 EasyTest™ ELISA Kit

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Human PF-4 EasyTest™ ELISA Kit
Human PF-4 EasyTest ELISA Standard Curve

Human PF-4 EasyTest™ ELISA Kit

Catalog No : 1010128


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SKU: 1010128. Category: .

Human PF-4 EasyTest™ ELISA Kit Features

Product Name           Human PF-4 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   6 pg/mL
Assay Range                15.6-1000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               PF4, CXCL4, SCYB4
Gene ID                         5196
Swissprot ID               P02776

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   118108-125
Plasma   121110-132

Linearity

Sample TypeDilution% of Expected
Serum1:2117
1:4102
1:887
Plasma1:2121
1:4105
1:881

Standard Curve: Human PF-4 EasyTest™ ELISA Kit

Human PF-4 EasyTest ELISA Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human PF-4 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine ligand 4 (CXCL4). It is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts by interacting with a splice variant of the chemokine receptor CXCR3, known as CXCR3B. PF4 probably has a role in inflammation and wound repair.

The heparin:PF4 complex is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. PF4 autoantibodies have also been found in patients with thrombosis and features resembling HIT but no prior administration of heparin.

Related Products

Human PF-4 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human PF-4 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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