Human PDGF R beta ELISA Kit

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Human PDGF R beta ELISA Kit
Human PDGF R beta ELISA Kit Standard Curve

Human PDGF R beta ELISA Kit

Catalog No : 3010126


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SKU: 3010126. Category: .

Human PDGF R beta ELISA Kit Features

Product Name            Human PDGF R beta ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15 pg/ml
Assay Range               24.7-18000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT
Gene ID                        5159
Swissprot ID              P09619 

 

Recovery

Sample Type    Average % RecoveryRange %
Serum10392-117
Plasma9889-107
Cell culture media9182-112

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:476
1:892
Plasma1:2102
1:482
1:894
Cell culture media1:2111
1:481
1:893

Standard Curve: Human PDGF R beta ELISA Kit

Human PDGF R beta ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

PDGF isoforms exert their cellular effects by binding to the structurally similar receptor tyrosine kinases (RTK), PDGF R alpha (CD140a) and PDGF R beta (CD140b). PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD and PDGF-AB isofoms bind the two distinct cell surface PDGF receptors with different affinities. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of RTK which are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and heterodimerization leading to signal transduction. The expression of PDGF alpha and beta receptors is independently regulated in various cell types.

Related Products

Human PDGF-AA ELISA Kit

Human PDGF-AB ELISA Kit

Human PDGF-BB EasyTest™ ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human PDGF R beta ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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