Human PDGF-AB EasyTest™ ELISA Kit

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Human PDGF-AB EasyTest™ ELISA Kit
Human PDGF-AB EasyTest ELISA Kit Standard Curve

Human PDGF-AB EasyTest™ ELISA Kit

Catalog No : 1010125


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SKU: 1010125. Category: .

Human PDGF-AB EasyTest™ ELISA Kit Features

Product Name           Human PDGF-AB EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubation
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   20 pg/mL
Assay Range                46.9-3000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis
Gene ID                         5155
Swissprot ID               P01127

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   8077-91
Plasma   7976-85

Linearity

Sample TypeDilution% of Expected
Serum1:288
1:487
1:891
Plasma1:2111
1:489
1:891

Standard Curve: Human PDGF-AB EasyTest™ ELISA Kit

Human PDGF-AB EasyTest ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human PDGF-AB mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

The term ‘PDGF’ refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. There are four members of the PDGF family – PDGF A, PDGF B, PDGF C and PDGF D.

Platelet-derived growth factor AB (PDGF-AB) is a disulfide-linked heterodimer of two chains termed subunit A and B, which form different members of the PDGF family including the homodimers PDGF-AA and PDGF-BB. PDGF-AB is synthesized by megakaryocytes and stored in alpha granules in platelets, from which it is released upon stimulation with thrombin or other signals related to inflammation and injury (IL-1, IL-6, TNF-α). PDGF-AB is also produced by macrophages, endothelial cells, fibroblast, and is present in the nervous system and in muscles.

By binding PDGF receptors alpha and beta (PDGFR-α and PDGFR-β), PDGF-AB acts as a potent mitogen on several mesenchymal-derived cells, including fibroblasts, smooth muscle cells, and cells of the connective tissue. PDGF-AB is not released to the circulation, but acts in the tissues in an autocrine and paracrine fashion. It is involved in hyperplasia, chemotaxis, wound healing, in the development of the nervous system, and in osteogenic differentiation.

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Human PDGF-AB ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human PDGF-AB EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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