Human PDGF-AA EasyTest™ ELISA Kit

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Human PDGF-AA EasyTest™ ELISA Kit
Human PDGF-AA EasyTest ELISA Standard Curve

Human PDGF-AA EasyTest™ ELISA Kit

Catalog No : 1010124


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SKU: 1010124. Category: .

Human PDGF-AA EasyTest™ ELISA Kit Features

Product Name           Human PDGF-AA EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubation
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   10 pg/mL
Assay Range                62.5-4000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               PDGFA, PDGF1
Gene ID                         5154
Swissprot ID               P04085

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   8077-88
Plasma   8176-91

Linearity

Sample TypeDilution% of Expected
Serum1:2102
1:479
1:887
Plasma1:2111
1:480
1:877

Standard Curve: Human PDGF-AA EasyTest™ ELISA Kit

Human PDGF-AA EasyTest ELISA Standard Curve

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human PDGF-AA mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

The term ‘PDGF’ refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. There are four members of the PDGF family – PDGF A, PDGF B, PDGF C and PDGF D.

PDGF-A and PDGF-B undergo intracellular activation during transport in the exocytic pathway and PDGF-C and PDGF-B are activated by extracellular proteases. Like PDGF-B, intracellular activation of PDGF-A, by removing the N-terminal sequence, is required for PDGF-A to bind to its receptor. PDGF-A can also be expressed by epithelial cells, smooth muscle cells, and neuronal progenitors.

PDGF-AA is well known as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). PDGF-AA can promote embryonic forebrain neural stem cells into O4-positive oligodendrocytes by activating Erk pathway. It also plays an important role in directing cell migration during embryonic development and postnatal migration response including wound healing. Chemotaxis ability of PDGF-AA has been shown on fibroblasts, smooth muscle cells, monocytes, and granulocytes.

Related Products

Human PDGF-AA ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human PDGF-AA EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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