Human Oncostatin M EasyTest™ ELISA Kit

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Human Oncostatin M EasyTest™ ELISA Kit
Human OSM EasyTest ELISA Kit Standard Curve

Human Oncostatin M EasyTest™ ELISA Kit

Catalog No : 1010121


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SKU: 1010121. Category: .

Product Features

Product Name           Human Oncostatin M EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   25 pg/mL
Assay Range                31.25-2000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               OSM
Gene ID                        5008
Swissprot ID              P13725

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   8779-95
Plasma   8577-92

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:497
1:889
Plasma1:287
1:489
1:892

Standard Curve: Human Oncostatin M EasyTest™ ELISA Kit

Human OSM EasyTest ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human OSM mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM. Endothelial cells possess a large number of OSM receptors. Stimulation of a primary endothelial culture (HUVEC) with hOSM results in delayed but prolonged upregulation of P-selectin, which facilitates leukocyte adhesion and rolling, necessary for their extravasation. OSM also promotes the production of IL-6 from these cells.

Related Products

Human Oncostatin M ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Oncostatin M EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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