Product Name Human Oncostatin M EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity 25 pg/mL
Assay Range 31.25-2000pg/mL
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names OSM
Gene ID 5008
Swissprot ID P13725
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Standard Curve: Human Oncostatin M EasyTest™ ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Bioaim human OSM mix
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM. Endothelial cells possess a large number of OSM receptors. Stimulation of a primary endothelial culture (HUVEC) with hOSM results in delayed but prolonged upregulation of P-selectin, which facilitates leukocyte adhesion and rolling, necessary for their extravasation. OSM also promotes the production of IL-6 from these cells.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Oncostatin M EasyTest™ ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit