Human NRG1-beta 1 EasyTest™ ELISA Kit

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Human NRG1-beta 1 EasyTest™ ELISA Kit
Human NRG1-beta 1 ELISA Kit Standard Curve

Human NRG1-beta 1 EasyTest™ ELISA Kit

Catalog No : 1010012


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SKU: 1010012. Category: .

Product Features

Product Name           Human NRG1-beta 1 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   200pg/mL
Assay Range               937.5-60000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               NRG1; GGF; HGL; HRGA; NDF; SMDF
Swissprot ID              Q02297

 

Recovery

Sample Type    Average % RecoveryRange %
Serum    123118-131
Plasma    126124-138

Linearity

Sample TypeDilution% of Expected
Serum1:295
1:490
1:896
Plasma1:292
1:483
1:881

Standard Curve: Human NRG1-beta 1 EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human NRG1-beta 1 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. NRG1 isoforms can be classified into three major subtypes. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human NRG1-beta 1 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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