Human Leptin ELISA Kit

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Human Leptin ELISA Kit

Human Leptin ELISA Kit

Catalog No : 3010021


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SKU: 3010021. Category: .

Product Features

Product Name           Human Leptin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   < 1pg/ml
Assay Range               1.6-400pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names              LEP, OB, OBS
Gene ID                       3952
Swissprot ID             P41159

 

Recovery

Sample Type    Average % RecoveryRange %
Serum  9482-103
Plasma  9284-101
Cell culture media  9587-105

Linearity

Sample TypeDilution% of Expected
Serum1:292
1:495
1:889
Plasma1:293
1:491
1:887
Cell culture media1:291
1:493
1:894

Standard Curve: Human Leptin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background: Leptin /OB

Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. It is opposed by the actions of the hormone ghrelin, the “hunger hormone”. Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. In obesity, a decreased sensitivity occurs, resulting in an inability to detect satiety despite high energy stores. Although regulation of fat stores is deemed to be the primary function, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have its receptors. Many of these additional functions are yet to be defined.

 Related Products

 Custom human Leptin R ELISA Kit

Mouse Leptin ELISA Kit

Rat Leptin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Leptin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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