Human IL-2 EasyTest™ ELISA Kit

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Human IL-2 EasyTest™ ELISA Kit
Human IL-2 ELISA Kit STandard Curve

Human IL-2 EasyTest™ ELISA Kit

Catalog No : 1010074


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SKU: 1010074. Category: .

Product Features

Product Name           Human IL-2 EasyTest™ ELISA Kit
Assay Type:                  Solid Phase Sandwich ELISA
Format:                          96-well strip plate
Assay Process:            One wash step, two incubations
Assay Length:             1.5 hours
Sample Volume:        Serum , Plasma, 50µL
Detection Method:   Colorimetric
Sensitivity:                  < 1pg/mL
Assay Range:               93.8-6000pg/mL
Reactivity:                    Human
Cross-Reactivity:      No cross-reactivity observed with available related molecules
Gene Names:              IL2; TCGF; lymphokine
Gene ID                        3558
Swissprot ID              P60568

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   111105-125
Plasma   10793-116

Linearity

Sample TypeDilution% of Expected
Serum1:2102
1:4118
1:898
Plasma1:2105
1:4118
1:8116

Standard Curve: Human IL-2 EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human IL-2 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells that are responsible for immunity. IL-2 is part of the body’s natural response to microbial infection, and in discriminating between foreign (“non-self”) and “self”. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. IL-2  is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IL-2 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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