Human IL-1ra ELISA Kit

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Human IL-1ra ELISA Kit
Human IL-1 ra ELISA Kit Standard Curve

Human IL-1ra ELISA Kit

Catalog No : 3010022


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SKU: 3010022. Category: .

Human IL-1ra ELISA Kit Features

Product Name           Human IL-1ra ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   30pg/ml
Assay Range               41.1-30000pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names              IL1RN, IL1F3, IL1RA
Gene ID                       3557
Swissprot ID             P18510

 

Recovery

Sample Type    Average % RecoveryRange %
Serum  9787-106
Plasma  9989-108
Cell culture media  9687-105

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:497
1:8101
Plasma1:2102
1:495
1:893
Cell culture media1:2101
1:494
1:891

Standard Curve: Human IL-1ra ELISA Kit

Human IL-1 ra ELISA Kit Standard Curve

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IL-1ra /IL-1F3 (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. It binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Related Products

Mouse IL-1ra EasyTest™ ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IL-1ra ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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