Human IL-1F6 ELISA Kit

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Human IL-1F6 ELISA Kit
Human IL-1F6 ELISA Kit

Human IL-1F6 ELISA Kit

Catalog No : 3010496


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SKU: 3010496. Category: .

Human IL-1F6 ELISA Kit Features

Product Name           Human IL-1F6 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.6 ng/mL
Assay Range                0.82-200 ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names                IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6
Gene ID                         27179
Swissprot ID               Q9UHA7

Recovery

Sample Type    Average % RecoveryRange %
Serum   7870-87
Plasma  7769-81
cell culture media   9182-101

Linearity

Sample TypeDilution% of Expected
Serum1:2114
1:4122
1:8111
Plasma1:2107
1:4117
1:898
cell culture media1:299
1:4102
1:8101

 Standard Curve: Human IL-1F6 ELISA Kit

Human IL-1F6 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon, is a member of the IL-1 family. IL-36 alpha is an 18 22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N linked glycosylation sites.

It can be released in response to LPS and the cell ATP induced activation of the P2X7 receptor. IL 36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells.

The receptor for IL 36 alpha is a combination of IL 1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL 1 RAcP. IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL 1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IL-1F6 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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