Human IL-18 R alpha ELISA Kit

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Human IL-18 R alpha ELISA Kit

Human IL-18 R alpha ELISA Kit

Catalog No : 3010493


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SKU: 3010493. Category: .

Human IL-18 R alpha ELISA Kit Features

Product Name           Human IL-18 R alpha ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   25 pg/mL
Assay Range                28.7-7000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names                IL18R1, CD218a, CDw218a, IL-1Rrp, IL18RA, IL1RRP
Gene ID                         8809
Swissprot ID               Q13478

Recovery

Sample Type    Average % RecoveryRange %
Serum   10494-117
Plasma  9588-106
cell culture media   9890-110

Linearity

Sample TypeDilution% of Expected
Serum1:288
1:486
1:887
Plasma1:297
1:487
1:898
cell culture media1:299
1:4102
1:8101

 Standard Curve: Human IL-18 R alpha ELISA Kit

Human IL-18 R alpha ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Interleukin-18 receptor 1 (IL-18R1), CDw218a, is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (IL-1 R5 and IL-1 Rrp) and IL-18 R beta ( IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IL-18 R alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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