Human IFN-gamma ELISA Kit

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Human IFN-gamma ELISA Kit
Human IFN-gamma ELISA Kit Standard Curve

Human IFN-gamma ELISA Kit

Catalog No : 3010007


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SKU: 3010007. Category: .

Product Features

Product Name           Human IFN-gamma ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10pg/mL
Assay Range               20.6-15000pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IFNG, IFG, IFI
Gene ID                         3458
Swissprot ID               P01579

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   8983-104
Plasma  8684-101
cell culture media   9385-102

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:495
1:891
Plasma1:296
1:497
1:893
cell culture media1:296
1:489
1:895

Standard Curve: Human IFN-gamma ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IFNγ, or type II interferon, is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFNγ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNγ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

Related Products

Human IFN-gamma EasyTest™ ELISA Kit

Mouse IFN-gamma EasyTest™ ELISA kit

Canine IFN-gamma ELISA Kit

Porcine IFN-gamma ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human IFN-gamma ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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