Human HPRG ELISA Kit

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Human HPRG ELISA Kit
Human HPRG ELISA Kit

Human HPRG ELISA Kit

Catalog No : 3010611


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SKU: 3010611. Category: .

Human HPRG ELISA Kit Features

Product Name           Human HPRG ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   1.2 ng/mL
Assay Range                1.23-300 ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               HRG, HPRGP, THPH11
Gene ID                         3273
Swissprot ID               P04196

Recovery

Sample Type    Average % RecoveryRange %
Serum   8071-90
Plasma  7871-86
cell culture media   7569-84

Linearity

Sample TypeDilution% of Expected
Serum1:290
1:4120
1:8116
Plasma1:297
1:4119
1:8112
cell culture media1:282
1:479
1:878

 Standard Curve: Human HPRG ELISA Kit

Human HPRG ELISA Kit

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region. Five distinct domains are recognized in the mature molecule. Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules, hemostatic molecules, and immune system components. About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++ dependent manner, it is converted to plasmin by tPA. HPRG also shows antiangiogenic activity on endothelial cells.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human HPRG ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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