Human GLP-1 (1-37a) ELISA Kit

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Human GLP-1 (1-37a) ELISA Kit

Human GLP-1 (1-37a) ELISA Kit

Catalog No : 3010614

SKU: 3010614. Category: .

Human GLP-1 (1-37a) ELISA Kit Features

Product Name           Human GLP-1 (1-37a) ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   2 pg/ml
Assay Range               2.3-150 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               GCG; GLP1; GLP2; GRPP
Gene ID                         2641
Swissprot ID               P01275


Sample Type    Average % RecoveryRange %
Serum  10884-133
Plasma  10381-127
cell culture media  10582-125


Sample TypeDilution% of Expected
cell culture media1:2135

Standard Curve: Human GLP-1 (1-37a) ELISA Kit

Human GLP-1 (1-37a) ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.


Glucagon-like peptide-1 (GLP-1) is an incretin derived from the transcription product of the proglucagon gene. The biologically active forms of GLP-1 are: GLP-1-(7-37) and GLP-1-(7-36) NH2. Those peptides result from selective cleavage of the proglucagon molecule.

GLP-1 secretion by ileal L cells is dependent on the presence of nutrients in the lumen of the small intestine. The secretagogues of this hormone include major nutrients like carbohydrate, protein and lipid. Once in the circulation, GLP-1 has a half-life of less than 2 minutes, due to rapid degradation by the enzyme dipeptidyl peptidase-4. It is a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. GLP-1 appears to restore the glucose sensitivity of pancreatic β-cells, with the mechanism possibly involving the increased expression of GLUT2 and glucokinase. GLP-1 is also known to inhibit pancreatic β-cell apoptosis and stimulate the proliferation and differentiation of insulin-secreting β-cells. In addition, GLP-1 inhibits gastric secretion and motility. This delays and protracts carbohydrate absorption and contributes to a satiating effect.

Related Products

Human GLP-1 (7-36a) ELISA Kit


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human GLP-1 (1-37a) ELISA Kit is for Research Use Only.


1 Plate Kit, 2 Plate Kit, 5 Plate Kit


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