Human Cystatin C ELISA Kit

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Human Cystatin C ELISA Kit
Human Cystatin C ELISA Kit Standard Curve

Human Cystatin C ELISA Kit

Catalog No : 3010052


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SKU: 3010052. Category: .

Human Cystatin C ELISA Kit Features

Product Name           Human Cystatin C ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   30 pg/ml
Assay Range               31.3-2000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CST3; ARMD11; HEL-S-2
Gene ID                        1471
Swissprot ID              P01034

 

Recovery

Sample Type    Average % RecoveryRange %
Serum 119106-132
Plasma 120110-136
Cell culture media 9990-107

Linearity

Sample TypeDilution% of Expected
Serum1:289
1:485
1:887
Plasma1:290
1:489
1:893
Cell culture media1:298
1:492
1:887

Standard Curve: Human Cystatin C ELISA Kit

Human Cystatin C ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer’s disease. In humans, all cells with a nucleus produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases and probably one of the most important extracellular inhibitors of cysteine proteases. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis.

Related Products

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Cystatin C ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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