Human Cystatin C EasyTest™ ELISA Kit

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Human Cystatin C EasyTest™ ELISA Kit
Human Cystatin C EasyTest ELISA Kit standard curve

Human Cystatin C EasyTest™ ELISA Kit

Catalog No : 1010052


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SKU: 1010052. Category: .

Product Features

Product Name           Human Cystatin C EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   45 pg/mL
Assay Range                78.2-5000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CST3; ARMD11; HEL-S-2
Gene ID                         1471
Swissprot ID               P01034

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   9284-101
Plasma   9589-102

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:496
1:898
Plasma1:2102
1:4101
1:889

Standard Curve: Human Cystatin C EasyTest™ ELISA Kit

Human Cystatin C EasyTest ELISA Kit standard curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human Cystatin C mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR). Serum levels of cystatin C are a more precise test of kidney function than serum creatinine levels. Cystatin C levels are less dependent on age, sex, race and muscle mass compared to creatinine. As opposed to previous claims, cystatin C has been found to be influenced by body composition. It has been suggested that cystatin C might predict the risk of developing chronic kidney disease.Studies have also investigated cystatin C as a marker of kidney function in the adjustment of medication dosages.

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Human Cystatin C ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Cystatin C EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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