Product Name Human Cystatin C EasyTest™ ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50µL
Detection Method Colorimetric
Sensitivity 45 pg/mL
Assay Range 78.2-5000pg/mL
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names CST3; ARMD11; HEL-S-2
Gene ID 1471
Swissprot ID P01034
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Standard Curve: Human Cystatin C EasyTest™ ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Bioaim human Cystatin C mix
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR). Serum levels of cystatin C are a more precise test of kidney function than serum creatinine levels. Cystatin C levels are less dependent on age, sex, race and muscle mass compared to creatinine. As opposed to previous claims, cystatin C has been found to be influenced by body composition. It has been suggested that cystatin C might predict the risk of developing chronic kidney disease.Studies have also investigated cystatin C as a marker of kidney function in the adjustment of medication dosages.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Cystatin C EasyTest™ ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit