Human CD163 ELISA Kit

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Human CD163 ELISA Kit
Human CD163 ELISA Kit Standard Curve

Human CD163 ELISA Kit

Catalog No : 3010044


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SKU: 3010044. Category: .

Human CD163 ELISA Kit Features

Product Name            Human CD163 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   15 pg/ml
Assay Range               32.8-8000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CD163; M130; MM130; SCARI1
Gene ID                         9332
Swissprot ID               Q86VB7

Recovery

Sample Type    Average % RecoveryRange %
Serum 10298-112
Plasma 10597-118
Cell culture media 9589-102

Linearity

Sample TypeDilution% of Expected
Serum1:292
1:488
1:890
Plasma1:289
1:492
1:891
Cell culture media1:291
1:482
1:887

Standard Curve: Human CD163 ELISA Kit

Human CD163 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CD163 is the high affinity scavenger receptor for the hemoglobin haptoglobin complex and in the absence of haptoglobin – with lower affinity – for hemoglobin alone. It has also been shown to mark cells of monocyte/macrophage lineage. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human CD163 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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