Human BMP-5 ELISA Kit

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Human BMP-5 ELISA Kit
Human BMP-5 ELISA Kit Standard Curve

Human BMP-5 ELISA Kit

Catalog No : 3010158


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SKU: 3010158. Category: .

Human BMP-5 ELISA Kit Features

Product Name            Human BMP-5 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   150 pg/mL
Assay Range                0.25-60 ng/mL
Reactivity                      Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               BMP5
Gene ID                         653
Swissprot ID              P22003

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   9888-115
Plasma   9581-103
Cell culture media   8580-94

Linearity

Sample TypeDilution% of Expected
Serum1:296
1:480
1:893
Plasma1:2101
1:483
1:897
Cell culture media1:298
1:490
1:892

Standard Curve: Human BMP-5 ELISA Kit

Human BMP-5 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. BMP5 may play a role in certain cancers. Like other BMP’s BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function.

Related Products

Human BMP-2 ELISA Kit

Human BMP-4 ELISA Kit

Human BMP-6 ELISA Kit

Human BMP-7 ELISA Kit

Human BMP-9 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human BMP-5 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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