Human BMP-2 ELISA Kit

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Human BMP-2 ELISA Kit
Human BMP-2 ELISA Kit Standard Curve

Human BMP-2 ELISA Kit

Catalog No : 3010004


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SKU: 3010004. Category: .

Product Features

Product Name            Human BMP-2 ELISA Kit
Assay Type                    Solid Phase Sandwich ELISA
Format:                          96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   20pg/mL
Assay Range                46.9-3000pg/ml
Reactivity                     Human
Cross-Reactivity       Around 1% cross-reactivity with human BMP-3, BMP-4, BMP-5, BMP-6 or BMP-7 was
                                           observed.
Gene Names               BMP2; BDA2A
Gene ID                         650
Swissprot ID               P12643

 

Recovery

Sample Type    Average % RecoveryRange %
Serum  10189-120
Plasma   9692-102
cell culture media   9483-105

Linearity

Sample TypeDilution% of Expected
Serum1:280
1:492
1:898
Plasma1:2110
1:4103
1:896
cell culture media1:295
1:497
1:896

Standard Curve: Human BMP-2 ELISA Kit

 Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. BMP-2 and BMP-7 are osteoinductive BMPs: they have been demonstrated to potently induce osteoblast differentiation in a variety of cell types.

Related Products

Human BMP-4 ELISA Kit

Human BMP-5 ELISA Kit

Human BMP-6 ELISA Kit

Human BMP-7 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human BMP-2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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