Product Name Human BMP-2 ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format: 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Assay Range 46.9-3000pg/ml
Cross-Reactivity Around 1% cross-reactivity with human BMP-3, BMP-4, BMP-5, BMP-6 or BMP-7 was
Gene Names BMP2; BDA2A
Gene ID 650
Swissprot ID P12643
|Sample Type||Average % Recovery||Range %|
|cell culture media||94||83-105|
|Sample Type||Dilution||% of Expected|
|cell culture media||1:2||95|
Standard Curve: Human BMP-2 ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Bone morphogenetic protein 2 or BMP-2 belongs to the TGF-β superfamily of proteins. BMP-2 like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is also involved in cardiac cell differentiation and epithelial to mesenchymal transition. BMP-2 and BMP-7 are osteoinductive BMPs: they have been demonstrated to potently induce osteoblast differentiation in a variety of cell types.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human BMP-2 ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit