Human bFGF EasyTest™ ELISA Kit

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Human bFGF EasyTest™ ELISA Kit
Human bFGF Easytest ELISA Kit Standard Curve

Human bFGF EasyTest™ ELISA Kit

Catalog No : 1010037


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SKU: 1010037. Category: .

Product Features

Product Name           Human bFGF EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   25 pg/mL
Assay Range                31.3-2000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               BFGF; FGF-2; FGFB; HBGF-2
Gene ID                         2247
Swissprot ID               P09038

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   9182-101
Plasma   9485-103

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:492
1:891
Plasma1:295
1:496
1:892

Standard Curve: Human bFGF EasyTest™ ELISA Kit

Human bFGF Easytest ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human bFGF mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

FGF basic (FGF-2) is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. Its bioactivity is modulated by a number of other binding partners including heparin, FGF-BP, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, PDGF, and CXCL4/PF4. In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples.

bFGF has been shown in preliminary animal studies to protect the heart from injury associated with a heart attack, reducing tissue death and promoting improved function after reperfusion. Recent evidence has shown that low levels of FGF2 play a key role in the incidence of excessive anxiety.

Related Products

Human bFGF ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human bFGF EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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