Human beta-NGF ELISA Kit

Human BMP-2 ELISA Kit
Human BMP-2 ELISA Kit
April 8, 2016
Human CD26 ELISA Kit
Human CD26 ELISA Kit
April 8, 2016
Show all
Human beta-NGF ELISA Kit
Human beta-NGF ELISA Kit Standard Curve

Human beta-NGF ELISA Kit

Catalog No : 3010005


Clear
SKU: 3010005. Category: .

Human beta-NGF ELISA Kit Features

Product Name           Human beta-NGF ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   5pg/mL
Assay Range               6.9-5000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               NGF; Beta-NGF; HSAN5B
Gene ID                        4803
Swissprot ID              P01138

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   11096-116
Plasma   10592-113
cell culture media   10290-111

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:4105
1:8101
Plasma1:2117
1:4113
1:898
cell culture media1:2102
1:4106
1:897

Standard Curve: Human beta-NGF ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Nerve growth factor (NGF) as a 7S complex composed of three non- covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases, while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. NGF is critical for the survival and maintenance of sympathetic and sensory neurons, as they undergo apoptosis in its absence. NGF binds with at least two classes of receptors: the tropomyosine receptor kinase A (TrkA) and low-affinity NGF receptor (LNGFR/p75NTR). When NGF binds to the TrkA receptor, it drives the homodimerization of the receptor, which in turn causes the autophosphorylation of the tyrosine kinase segment. This leads to the activation of PI 3-kinase, ras, and PLC signaling pathways.

Related Products

Human beta-NGF EasyTest™ ELISA Kit

Human NGF R ELISA Kit

Mouse beta-NGF ELISA Kit

Rat beta-NGF ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human beta-NGF ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Human beta-NGF ELISA Kit”