Human ApoA2 ELISA Kit

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Human ApoA2 ELISA Kit

Human ApoA2 ELISA Kit

Catalog No : 3010384


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SKU: 3010384. Category: .

Human ApoA2 ELISA Kit Features

Product Name            Human ApoA2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                    25 pg/mL
Assay Range                0.027-20 ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               APOA2, Apo-AII, ApoA-II, apoAII
Gene ID                         336
Swissprot ID               P02652

Recovery

Sample Type    Average % RecoveryRange %
Serum   11790-130
Plasma   12298-134
cell culture media   11092-128

Linearity

Sample TypeDilution% of Expected
Serum1:2111
1:487
1:885
Plasma1:2106
1:497
1:891
cell culture media1:2108
1:498
1:895

 Standard Curve: Human ApoA2 ELISA Kit

Human ApoA2 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Apolipoprotein A-II is a protein that is encoded by the APOA2 gene. It is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. APOA2 has been shown to interact with PLTP. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human ApoA2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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