Human Angiopoietin-4 ELISA Kit

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Human Angiopoietin-4 ELISA Kit
Human Angiopoietin-4 ELISA Kit Standard Curve

Human Angiopoietin-4 ELISA Kit

Catalog No : 3010154


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SKU: 3010154. Category: .

Product Features

Product Name            Human Angiopoietin-4 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   200 pg/mL
Assay Range                0.27-200 ng/mL
Reactivity                      Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ANGPT4, ANG3, ANG4
Gene ID                        51378
Swissprot ID              Q9Y264

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   10188-124
Plasma   8072-91
Cell culture media   11095-128

Linearity

Sample TypeDilution% of Expected
Serum1:2110
1:497
1:8105
Plasma1:2121
1:4123
1:8117
Cell culture media1:2117
1:4108
1:8116

Standard Curve: Human Angiopoietin-4 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The mechanism by which they contribute to angiogenesis is thought to involve regulation of endothelial cell interactions with supporting perivascular cells. The protein encoded by this gene functions as an agonist and is an angiopoietin.

Related Products

Human Angiopoietin-1 EasyTest™ ELISA Kit

Human Angiopoietin-2 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human Angiopoietin-4 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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