Product Name Human Amyloid beta 1-40 ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 0.10 ng/mL
Assay Range 0.14-100 ng/mL
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names APP, AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, preA4,
Gene ID 351
Swissprot ID P05067
|Sample Type||Average % Recovery||Range %|
|cell culture media||102||82-132|
|Sample Type||Dilution||% of Expected|
|cell culture media||1:2||132|
Standard Curve: Human Amyloid beta 1-40 ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Amyloid beta (Aβ or Abeta) denotes peptides of 36–43 amino acids. The peptides derive from the amyloid precursor protein (APP), which is cleaved by beta secretase and gamma secretase to yield Aβ. Aβ molecules can aggregate to form flexible soluble oligomers which may exist in several forms. It is now believed that certain misfolded oligomers can induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells.
Aβ is the main component of amyloid plaques (extracellular deposits found in the brains of patients with Alzheimer’s disease).Similar plaques appear in some variants of Lewy body dementia and in inclusion body myositis, while Aβ can also form the aggregates that coat cerebral blood vessels in cerebral amyloid angiopathy. The plaques are composed of a tangle of regularly ordered fibrillar aggregates called amyloid fibers, a protein fold shared by other peptides such as the prionsassociated with protein misfolding diseases.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Amyloid beta 1-40 ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit