Human AMICA ELISA Kit

Human Alpha-2 M ELISA Kit
Human Alpha-2 M ELISA Kit
July 13, 2017
Human Aminopeptidase N ELISA Kit
July 14, 2017
Show all
Human AMICA ELISA Kit
Human AMICA ELISA Kit

Human AMICA ELISA Kit

Catalog No : 3010346


Clear
SKU: 3010346. Category: .

Human AMICA ELISA Kit Features

Product Name           Human AMICA ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.40 ng/mL
Assay Range                0.41-100 ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               JAML, AMICA, AMICA1, CREA7-1, CREA7-4, Gm638
Gene ID                         120425
Swissprot ID               Q86YT9

Recovery

Sample Type    Average % RecoveryRange %
Serum   11497-128
Plasma   10990-130
cell culture media   8985-101

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:490
1:895
Plasma1:2102
1:487
1:891
cell culture media1:280
1:4102
1:8105

 Standard Curve: Human AMICA ELISA Kit

Human AMICA ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

ALCAM, activated leukocyte cell adhesion molecule, also known as CD166, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human AMICA ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

Reviews

There are no reviews yet.

Be the first to review “Human AMICA ELISA Kit”