Human AFP ELISA Kit

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Human AFP ELISA Kit
Human AFP ELISA Kit Standard Curve

Human AFP ELISA Kit

Catalog No : 3010600


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SKU: 3010600. Category: .

Human AFP ELISA Kit Features

Product Name           Human AFP ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   5 pg/mL
Assay Range                7.4-1800 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               AFP, AFPD, FETA, HPAFP
Gene ID                         174
Swissprot ID               P02771

Recovery

Sample Type    Average % RecoveryRange %
Serum   130119-141
Plasma   128114-137
cell culture media   127113-136

Linearity

Sample TypeDilution% of Expected
Serum1:2107
1:495
1:8101
Plasma1:2109
1:489
1:891
cell culture media1:299
1:480
1:882

 Standard Curve: Human AFP ELISA Kit

Human AFP ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. It is a major plasma protein produced by the yolk sac and the liver during fetal development. AFP is thought to be the fetal form of serum albumin. It binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms.

AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown. Measurement of AFP is generally used in two clinical contexts. First, it is measured in pregnant women through the analysis of maternal blood or amniotic fluid as a screening test for certain developmental abnormalities. Second, serum AFP level is elevated in people with certain tumors, such as liver cancer.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human AFP ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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