GSK3a(Phospho-Ser21) Antibody

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GSK3b Antibody
GSK3a(Phospho-Ser21) Antibody
GSK3a(Phospho-Ser21) Antibody

GSK3a(Phospho-Ser21) Antibody

Catalog No : 11007


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SKU: 11007. Category: .

Product Name

GSK3a(Phospho-Ser21) Antibody

Host Species

Rabbit

Clonality

 Polyclonal

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Applications

WB IHC IF

Reactivity

Hu Ms Rt

Specificity

The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.

Immunogen

Peptide-KLH. Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.

Target Name

GSK3a

Alternative Name

Factor A; GSK-3 alpha; kinase GSK3-alpha

Modification

Phospho-Ser21

SDS-PAGE MW

51kd

Accession No

P49840; NP_063937.2

Concentration

1.0mg/ml

Formulation

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Background

Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.

Sizes

100ul, 50ul

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