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human SIGIRR ELISA Kit

Custom human SIGIRR ELISA Kit

Catalog No : 3010567


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SKU: 3010567. Category: .

Product Features

Product Name            Custom human SIGIRR ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Human
Cross-Reactivity       This ELISA antibody pair only recognizes human SIGIRR
Gene Names:              SIGIRR, IL-1R8, TIR8
Gene ID                         59307
Swissprot ID               Q6IA17

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI, IL-1 RII, IL-1 RAcP, ST2, IL 18 R alpha, IL-1 Rrp2, IL-18 R beta, IL-1RAPL 1 (TIGIRR 2/IL 1 R8), and TIGIRR-1 (IL-1 R9). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. The sequence of human SIGIRR predicts a 410 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 118 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL-1 R8, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members.

SIGIRR is widely expressed and is present in all cells and tissues examined. The ligand and signaling mechanism for SIGIRR has not been identified. It has been observed to modulate immune responses, colonic epithelial homeostasis and tumorigenesis. SIGIRR has been shown to interact with TRAF6.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom human SIGIRR ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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  • Manual
  • Assay procedure
  • MSDS