Custom human ICAM-2 ELISA Kit

human GASP-2 ELISA Kit
Custom human GASP-2 ELISA Kit
January 2, 2017
human IL-13 R alpha 1 ELISA Kit
Custom human IL-13 R alpha 1 ELISA Kit
January 2, 2017
Show all
human ICAM-2 ELISA Kit

Custom human ICAM-2 ELISA Kit

Catalog No : 3010474


Clear
SKU: 3010474. Category: .

Product Features

Product Name           Custom human ICAM-2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Human
Cross-Reactivity       This ELISA antibody pair only recognizes human ICAM-2
Gene Names:              ICAM2; CD102
Gene ID                         3384
Swissprot ID               P13598

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Intercellular adhesion molecule 2 (ICAM2), also known as CD102, is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2–9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 molecules regulate spermatid adhesion on Sertori cell on the apical side of the blood-testis barrier (towards the lumen), thus playing a major role in spermatogenesis. It may also play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has been shown to interact with EZR.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom human ICAM-2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

Reviews

There are no reviews yet.

Be the first to review “Custom human ICAM-2 ELISA Kit”

  • Manual
  • Assay procedure
  • MSDS