Canine IL-6 ELISA Kit

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Canine IL-6 ELISA Kit
Canine IL-6 ELISA Kit Standard Curve

Canine IL-6 ELISA Kit

Catalog No : 3060018


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SKU: 3060018. Category: .

Canine IL-6 ELISA Kit Features

Product Name            Canine IL-6 ELISA Kit
Assay Type                    Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   50 pg/ml
Assay Range               0.102-25 ng/ml
Reactivity                     Canine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IL6, IL-6
Gene ID                        403985
Swissprot ID              P41323

 

Recovery

Sample Type    Average % RecoveryRange %
Serum8072-90
Plasma7970-88
Cell culture media10799-112

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:489
1:886
Plasma1:295
1:487
1:883
Cell culture media1:296
1:484
1:889

Standard Curve: Canine IL-6 ELISA Kit

Canine IL-6 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130.

Recent work has shown that both upstream and downstream signalling pathways for IL-6 differ markedly between myocytes and macrophages. It appears that unlike IL-6 signalling in macrophages, which is dependent upon activation of the NFκB signalling pathway, intramuscular IL-6 expression is regulated by a network of signalling cascades, including the Ca2+/NFAT and glycogen/p38 MAPK pathways. Thus, when IL-6 is signalling in monocytes or macrophages, it creates a pro-inflammatory response, whereas IL-6 activation and signalling in muscle is totally independent of a preceding TNF-response or NFκB activation, and is anti-inflammatory.

Related Products

Human IL-6 EasyTest™ ELISA Kit

Mouse IL-6 ELISA Kit

Rat IL-6 ELISA Kit

Porcine IL-6 ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Canine IL-6 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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