Canine IFN-gamma ELISA Kit

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Canine IFN-gamma ELISA Kit
Canine IFN-gamma ELISA Kit Standard Curve

Canine IFN-gamma ELISA Kit

Catalog No : 3060007


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SKU: 3060007. Category: .

Canine IFN-gamma ELISA Kit Features

Product Name            Canine IFN-gamma ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   50 pg/ml
Assay Range               0.102-25 ng/ml
Reactivity                     Canine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IFNG, IFN-G, IFN-gamma
Gene ID                        403801
Swissprot ID              B6E117

 

Recovery

Sample Type    Average % RecoveryRange %
Serum8072-89
Plasma8274-92
Cell culture media7975-88

Linearity

Sample TypeDilution% of Expected
Serum1:287
1:4119
1:898
Plasma1:2105
1:4104
1:892
Cell culture media1:2112
1:4101
1:892

Standard Curve: Canine IFN-gamma ELISA Kit

Canine IFN-gamma ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It is secreted by T helper cells (specifically, Th1 cells), cytotoxic T cells (TC cells) and NK cells only. IFNγ is the only Type II interferon and it is serologically distinct from Type I interferons; it is acid-labile, while the type I variants are acid-stable. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. IFNγ also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation.

Related Products

Human IFN-gamma EasyTest™ ELISA Kit

Mouse IFN-gamma EasyTest™ ELISA kit

Porcine IFN-gamma ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Canine IFN-gamma ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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