Canine HGF ELISA Kit

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Canine HGF ELISA Kit
Canine HGF ELISA Kit Standard Curve

Canine HGF ELISA Kit

Catalog No : 3060082


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SKU: 3060082. Category: .

Canine HGF ELISA Kit Features

Product Name           Canine HGF ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.2 ng/ml
Assay Range               0.25-60 ng/ml
Reactivity                     Canine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               MET
Gene ID                        403441
Swissprot ID               Q867B7

Recovery

Sample Type    Average % RecoveryRange %
Serum 9580-113
Plasma 10186-119
Cell culture media 122110-132

Linearity

Sample TypeDilution% of Expected
Serum1:2116
1:4110
1:8104
Plasma1:2117
1:4121
1:8115
Cell culture media1:291
1:498
1:889

Standard Curve: Canine HGF ELISA Kit

Canine HGF ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

HGF is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis. HGF induces the proliferation and migration of epithelial cells, hepatocytes and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Canine HGF ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Canine (dog)

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