ELISA FAQs

ELISA FAQs

Q: How many samples can I test on one ELISA?

A: On each 96 well plate, an 8-point standard curve is run with duplicate wells. The remaining wells may then be used for samples (duplicates are strongly recommended). If the entire plate is used in one experiment, the remaining 80 wells will accommodate 40 samples in duplicate. If not run all at once, a new standard curve must be included with every run.

Q: Does your ELISA kit have a positive control sample?

A: The recombinant standard may serve as a positive control or may be used for spike recovery studies. Biological reference samples are not included in Bioaim ELISA kits.

Q: My kit was left out at room temperature for several days. Is it still good?

A: The kit may have diminished activity after an extended period at room temperature, but it is most likely still usable, especially if left out for only a small amount of time.

Q: What is your ELISA kit validation process?

A: At Bioaim we take our product quality very seriously. Every kit we produce is subjected to a battery of rigorous product-specific quality control tests and manufactured in compliance with GMP. We follow industrial guidelines to do precision, linearity and recovery tests for all ELISA kits.

Q: How do I prepare my samples?

A: Our tips for sample preparation can be found here:

Sample Preparation Tips

Q: What Dilution do you recommend for my samples?

• Serum and plasma - The recommended dilution ranges are indicated in the product manual, in Section VI (Reagent Preparation).
• Conditioned medium - The analyte levels depend entirely on the experimental conditions, thus optimal dilution factors must be determined empirically. However in general we recommend starting with no dilution or a 1:2 dilution of conditioned medium.
• Other body fluids (saliva, CSF, urine, tears, BAL fluid, milk, etc.) - Optimal dilution ranges vary widely and should be determined by the investigators.

Q: What is the difference between your normal sandwich ELISA and EasyTestTM ELISA?

A: Bioaim EasyTestTM ELISA only needs one wash step, so it can decreases variations from pipetting and wash and the whole process takes less than 2 hours. The detailed information can be found in “Bioaim EasyTestTM ELISA Technology” and EasyTestTM ELISA kit manuals.

Q: Do I need add detection antibody and HRP separately during experiment?

A: You don’t need to add detection antibody and HRP separately to your plate. All the conditions have been optimized and you just need to follow manuals to add samples and Bioaim mix together.

Q: what is the sensitivity of your EasyTestTM ELISA?

A: Normally, Bioaim EasyTestTM ELISA has the same sensitivity as our normal sandwich ELISA.

Q: Do I need any special steps to treat samples when use EasyTestTM ELISA kits?

A: No.